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Sysprep Server 2008r2 on production server

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I recently set up a new virtual host with three 08r2 Standard guest installations (DC, Database server, app/file server) but neglected to sysprep the base virtual before setting up the servers.

What issues would come up if I were to sysprep the database/app servers to generate new SID values? They are currently joined to the domain, both of them host file shares and a few internal applications. I mainly am not sure what data/options are removed/reset when you run sysprep on a server.

My hope is that I don't have to completely rebuild the servers from scratch.

Thank you


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