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Server 2003 on Premise DC stops replicating to AWS EC2 AD

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Hi Everyone, can you help me troubleshoot this issue that I have been having for the past weeks,

I have a server 2003 Primary and secondary Domain controllers that are replicating to each other fine, but the DC1 is also replicated to my Production  server on AWS EC2 AD that has been working fine for years now and I have all the recommended ports are open on the EC2 instance, but for the past week now, when I create a new domain user in DC1, it is not found in the EC2 instance server 2012.

 (not syncing /replicating.)

I will appreciate your speedy assistance ' with a clear and understandable step by step troubleshooting or solution.



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