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Terminal server issues Server 2008

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Hello we are running terminal services using server 2008. We have 2 nics installed in this server and terminal services configuration is set for "all network adapters configured with this protocol" Everything was working correctly and now the second nic won't connect. I can ping it and it responds, but when I try to connect via rdp the error message says

"Remote desktop can't connect to the remote computer for one of these reasons:

1) Remote access to the server is not enabled

2) The remote computer is turned off

3) The remote computer is not available on the network

If we do a netstat -na on the server it does not show that it is listening on port 3389 yet we have about 20 users connected via the first nic.

A wire shark capture from the client to the remote server shows 3 syn packets sent and no response back from the server. A wire shark capture on the server shows the 3 incoming syn packets and no response back to the client.

Any suggestions on what to check to try and figure this out are appreciated.


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